一株山羊支原体山羊肺炎亚种的分离鉴定与分子特征

从送检的山羊肺炎肺脏中成功分离到一株支原体，经过3次克隆纯化后进行生化试验、电镜观察、PCR及酶切、基因特征鉴定，结果显示分离物SD3属于山羊支原体山羊肺炎亚种成员。将培养物经气管接种2只山羊可引起1只山羊典型发病，体温升高至41．5℃，IgG和IgM抗体效价明显升高，其中IgG抗体变化与临床表现基本同步。剖检发现肺脏发生严重病变，并从中再次分离到该病原体。     

羊草与灰色赖草杂种F1再生体系的建立

对羊草(Leymus chinensis)与灰色赖草(Leymus cinereus)杂种F1幼穗进行离体培养,建立了杂种F1植株再生体系.结果表明:不含任何激素的MS培养基是最佳的分化培养基,分化成苗率为66%;2,4-D对愈伤组织的诱导及其质地的改造有重要作用;3.0 mg/L 2,4-D MS培养基愈伤组织诱导率最高,为68%;2.0 mg/L 2、4-D MS诱导的愈伤组织胚性最强;诱导率与分化率没有直线对应关系;再生体系的建立为杂种F1育性恢复的研究奠定了基础.     

花后温度和湿度条件对台农1号杧坐果率的影响

为探索杧果坐果率与开花后温度和湿度条件的关系,以台农1号杧为试材,应用生物统计学方法计算分析了80个温度和湿度自变量与不同时期坐果率的相关关系。结果表明:(1)花后14d的坐果率受温度和湿度条件影响最大。(2)花后7d两性花坐果率与花后1～6d平均空气相对湿度呈显著直线线性负相关,与花后1～2d、1～4d、1～6d的平均最高温度、平均温度、平均最低温度、>10℃积温无显著相关关系。(3)花后14d坐果率与花后6～10d的平均最高温度、4～10d平均温度、2～10d平均最低温度、4～14d>10℃积温呈显著或极显著的直线相关;与花后1～14d平均最高温度、平均温度呈显著的多项式线性相关;与花后1～14d平均空气相对湿度呈极显著的直线线性负相关。(4)花后25d坐果率与花后1～10d的平均最高温度、平均温度、>10℃积温以及花后1～14d平均最高温度呈显著的多项式线性相关。(5)有利于花后14d坐果的花后4～14d的下限平均温度为20.6℃,最适温度为24.7℃。     

Effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene in DLD1 colon cancer cells

AIM:To evaluate effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene and clarify the involved mechanisms in DLD1-TRAIL/R colon cancer cells. METHODS:Human colon cancer cell line DLD1-TRAIL/R cells that were resistant to TRAIL-expressing adenovector (Ad/gTRAIL) were treated with Ad/gTRAIL combined with different chemotherapeutic agents. Then, the cell viability was measured by MTT method, and apoptotic signaling conditions, including activation of caspase-3 and caspase-8, expression of Bax and Bcl-XL, were measured by Western blotting analysis. RESULTS:In vitro data showed that several chemotherapeutic agents, including 5-fluorouracil (5-FU) and mitomycin c (MMC), overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R colon cancer cells. The combination of Ad/gTRAIL and 5-FU effectively suppressed tumor growth in vivo in subcutaneous tumors established from DLD1-TRAIL/R cells. Further data showed that treatment with the combination of Ad/gTRAIL and 5-FU or MMC led to enhance the activation of caspase-3. Moreover, MMC but not 5-FU induced overexpression of Bax gene that was sufficient to overcome the resistance to TRAIL gene in DLD1-TRAIL/R cells. CONCLUSION:Chemotherapeutic agents, such as 5-FU and MMC, overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R cells. The candidate mechanisms for MMC but not 5-FU to overcome this resistance might involve the induction of over-expressed Bax protein in DLD1-TRAIL/R cells.     

Alteration of efferent system functions in noise-induced hearing loss patients

AIM:To investigate the changes of efferent system functions in noise-induced hearing loss (NIHL) patients and find out a method of clinic diagnosis for NIHL.METHODS:The amplitudes of distortion product otoacoustic emission were measured with and without contralateral broad-band noise using Celesta-503 otoacoustic emission analyzer. The efferent system functions were evaluated by the amplitudes of contralateral suppression.RESULTS:The contralateral suppressions in NIHL patients were significant lower than those in control group in low frequency (0.75 kHz and 1 kHz) of DP-gram and low level primary tone in 2 kHz input/output function. There were no significant differences in contralateral suppressions of 4 kHz and 6 kHz input/output function between NIHL patients and control group.CONCLUSION:The contralateral suppressions in NIHL patients are lower than those in normal hearing subjects and the measurements of efferent system functions could be used in early detection and clinic diagnosis for NIHL.     

青海河湟地区水资源综合开发与区域经济发展研究

本文分析了河湟地区水资源特征、开发利用现状及存在的问题,在此基础上,论述了河湟地区水资源开发利用与区域经济发展模式。研究表明:加快开发黄河水能资源,建立以水能资源开采业为主导的产业群发展;浅山地区因地制宜推广雨水集流利用工程,走出“水窑+科技+节水产业+生态”的发展模式;“引大济湟”工程有助于提高水资源可利用量,推进湟水流域全面发展。     

根结线虫在中国的新纪录种——西班牙根结线虫(Meloidogyne hispanica Hirschmann)

 A root-knot nematode, Meloidogyne hispanica Hirschmann, was first described and illustrated from roots of papaya in Danzhou, Hainan province in China. The perineal pattern of female was characterized by oval shaped to rectangular with a low dorsal arch; dorsal striae varied from fine and wavy to coarse; lateral lines forked with fringe-like striae between lines and phasmidial ducts distinctly appeared. Male had high and rounded head region with labial disc and medial lips fused to form elongate lip structures. Second-stage juve-nile had distinct body annules and stylet with rounded, posteriorly sloping knobs, and the tail terminus was slender with indistinct hyaline. This Chinese new record of M. hispanica had a unique esterase phenotype (S2-M1) which was different from that of other Meloidogyne species.     

基于SWOT分析的土地复垦模式研究——以克俄露天采矿区土地复垦模式为例

简要介绍了SWOT方法,然后将之应用于克俄露天矿区土地复垦模式的评价中,通过对比分析,将较为复杂的关系通过矩阵形式清晰地表现出来,指明了该复垦模式未来发展的战略方向。研究表明,SWOT方法在土地复垦模式发展方向的表达上是非常直观有效的,在逻辑上也是严密合理的。     

不同培养基对肠球菌的检测效果比较

肠球菌是食品、水、食品加工设备和食品生产环境等卫生状况的指示菌之一,对其进行检测的培养基种类较多。选用叠氮化钠葡萄糖肉汤和肠球菌肉汤对奶粉和水中肠球菌进行检测比较,结果表明,肠球菌肉汤具有绝对的优势,但在实际检测工作中,对不同样品进行检测时,应注意根据样品的性状合理选择培养基,以提高检测的速度和准确性。